The ability to generate 300mer oligos is a critical tool for researchers, enabling them to screen more than 70% of sequences identified in PFAM as human nuclear protein domains, which are potential effectors. Coupled with the exceptional precision, length, and uniformity of Twist Oligos, HT-recruit opens the door to protein domain characterization on a genome-wide scale.  

The use of CRISPR screening technology enables the interrogation of gene function at virtually any scale, especially in pooled screening applications. While genome-wide CRISPR screen libraries are easily accessible off-the-shelf, their lack of design flexibility means it is difficult to cost-effectively address specific research questions, thus limiting the applicability of these libraries. Twist Bioscience’s custom CRISPR guide libraries give you the flexibility to tailor your sgRNA library to your species, strain, and research question, all with the confidence that guides in your library will be precisely synthesized and equally represented.

Twist’s industry leading DNA synthesis platform generates oligo pools with unparalleled precision and uniformity. Beyond synthesis, Twist now offers an optimized cloning process that allows you to further avoid the tribulations of testing PCR amplification conditions, selecting the right polymerases and primer pairs, and designing a cloning workflow.