Libraries converted for methylation sequencing pose unique challenges to target enrichment because they are less complex than typical libraries. To overcome these obstacles, Twist optimized the Twist NGS Methylation Detection System with two key advancements: adaptive panel design and a methylation-specific blocker called Methylation Enhancer. This technical note outlines how these optimizations provide both flexibility and unprecedented control over the off-target rate in targeted methylation sequencing.

  Twist Bioscience’s proprietary semiconductor-based Twist Bioscience’s proprietary semiconductor-based synthetic DNA manufacturing process featuring a high-throughput silicon platform offers precision at a scale unavailable anywhere else. The innovative method of DNA production enables the manufacture of high-quality synthetic DNA, enabling customers to accelerate success. The Twist NGS Methylation Detection System provides a robust, end-to-end sample preparation solution for identifying methylated regions in the human genome. The workflow employs a unique enzymatic process from New England Biolabs® that is much less damaging to DNA, alongside Twist‘s Custom Methylation Panel design. Whether you are investigating cellular differentiation or screening liquid biopsies for cancer, the system offers the most efficient methylation detection available.