Custom CRISPR screens are an important tool in functional genomics, offer an economical way to answer specific questions that are not easily accomplished with off-the-shelf screens. However, custom screens require up front design and planning efforts to effectively execute. In this eBook you will discover ways to optimize and utilize custom CRISPR screens to get the most out of your research. You'll take a dive into the experimental design strategy for custom CRISPR screens, learn about the oligo pool properties that lead to success when building your custom guide library, and discover some of the innovations that custom CRISPR screens are driving today. By following the guidance in this eBook, you'll be able to streamline your screening process and speed up your research!

Prioritization of Antimicrobial Targets by CRISPR-based Oligo Recombineering