Validating a diagnostic cfDNA NGS assay

EN

English
Chinese
Español
Japanese

Support

Contact Us
FAQ
Match with an Expert

MY ORDERS

Open Orders
Drafts
Past Orders

Sign In

Genes
Oligo Pools
NGS
Variant Libraries
Antibodies
IVDR Solutions

Genes

START ORDER

ARRAYED GENES

Clonal Genes Upgraded Gene Fragments Demo Test NEW

POOLED GENES

Gene Pools Multiplexed Gene Fragments New Explore all Genes >

Sequence Design Workbench

Manufacturability Analyzer Codon Optimizer Vector toolbox

Learn more >

Oligo Pools

ORDER NOW

Cloned Oligo pools

Oligo Pools

Read our app note on Twist Oligo Pools for CRIPSR screens

Read our app note on Twist Oligo Pools for CRIPSR screens

NGS Target Enrichment Solutions

SHOP ALL NGS PRODUCTS

TARGET ENRICHMENT PANELS >

Custom Panel Exome 2.0 DNA CGP Panel RNA Exome Human Methylome Panel Shop All

TARGET ENRICHMENT SOLUTIONS & ENZYMES >

Fast Hybridization Solution Fast Hybridization Solution, Element Trinity Compatible Standard Hybridization Solution TrueAmp Polymerase Mix Shop all

LIBRARY PREPARATION SOLUTIONS >

TrueAmp Library Prep Kit PCR-Free WGS Library Prep Kit cfDNA Library Prep Kit with TrueAmp Polymerase Mix RNA Library Prep Kit EM-seq v2 Methylation Detection System Shop all

SYNTHETIC CONTROLS >

cfDNA Pan-Cancer Reference Standard v2 Respiratory Virus Controls Shop all

Twist TrueAmp Library Preparation Kit enables high uniformity across GC-rich, AT-rich, and repeat-dense regions. Learn More >

Twist TrueAmp Library Preparation Kit enables high uniformity across GC-rich, AT-rich, and repeat-dense regions. Learn More >

Variant Libraries

ORDER NOW

TCR Libraries NEW

Combinatorial Variant Library

Spread Out Low Diversity Libraries

Site Saturation Libraries

Enzyme Screening kits NEW

Watch how Twist builds variant libraries for your perfect application

Watch how Twist builds variant libraries for your perfect application

ORDER NOW

DISCOVERY

In vitro discovery In vivo discovery Antibody engineering and optimization Critical reagents and tool antibodies Licensing

PRODUCTION AND CHARACTERIZATION

Antibody production Antibody characterization Bispecific antibodies

Propel discovery with 6,000+ IgGs/week and full characterization data in as few as 15 days.

Propel discovery with 6,000+ IgGs/week and full characterization data in as few as 15 days.

Applications

NGS Workflow
AI Drug Discovery
Cancer Research
AgBio
Drug Discovery
Infectious Disease

NGS WORKFLOWS >

Target Enrichment Workflow Whole Genome Sequencing Whole Exome Sequencing RNA Sequencing Methylation Sequencing

Twist TrueAmp Library Preparation Kit enables high uniformity across GC-rich, AT-rich, and repeat-dense regions. Learn More >

Twist TrueAmp Library Preparation Kit enables high uniformity across GC-rich, AT-rich, and repeat-dense regions. Learn More >

TOOLS FOR AI DRUG DISCOVERY

Benefits Workflow Assay Suite Contact Us

Tools for Cancer Research and Clinical Oncology >

Whole Exome Sequencing Whole Genome Sequencing

Liquid Biopsy >

cfDNA Library Preparation Kit NEW cfDNA Pan-Cancer Reference Standards v2 NEW Minimal Residual Disease NEW

Learn more about MRD Express

Learn more about MRD Express

Tools for Drug Discovery

VHH Antibody Discovery Bispecific antibodies

Antibody Services

Antibody Discovery Antibody Optimization Pipeline & Licensing Partnership & Collaborations

ORDER NOW

Tools for Infectious Disease

SARS-CoV-2

SARS-CoV-2 Research Panel SARS-CoV-2 RNA Controls

Catalog Antibodies

Anti-ACE2 Anti-SARS-CoV-2 S1

Synthetic Controls

Monkeypox Virus Controls Respiratory Virus Controls

Fixed Panels

Comprehensive Viral Research Panel Respiratory Virus Research Panel

Check out our fixed-panels for respiratory virus research

Check out our fixed-panels for respiratory virus research

resources

Technical Resources
Webinar
Case Studies
Publications
Podcasts
Videos
Blog
FAQ
Codon Optimization NEW
MasterMix Calculator
Twist Wallet

COMPANY

Company
Technology
Investor Relations
Partnerships
Biopharma

About

Team

Careers

Investor Relations

Events

Contact

Blog

Corporate Responsibility

Learn More About NGS ProLab Certified Services For The Twist NGS Portfolio

Learn More About NGS ProLab Certified Services For The Twist NGS Portfolio

Overview

Ordering Platform

Twist API

Procurement Integrations

Overview

STOCK

Stock Quote Chart Analyst Coverage

News and Events

Press Releases Investor Calendar Presentations

Financials

Sec Filings

Governance

Governance Hightlights Commitee Composition

Resources

Email Alerts IR Contacts

NGS Prolab

Channel Partners and Distributors

Start Order

- Libraries

Products

IVDR Solutions

Support

Chinese
English
Japanese
Español

Support

Contact Us
FAQ
Match with an Expert

MY ORDERS

Open Orders
Drafts
Past Orders

Genes

ARRAYED GENES
POOLED GENES
Sequence Design Workbench

Oligo Pools

Cloned Oligo pools
Oligo Pools

NGS

TARGET ENRICHMENT PANELS >
TARGET ENRICHMENT SOLUTIONS & ENZYMES >
LIBRARY PREPARATION SOLUTIONS >
SYNTHETIC CONTROLS >

Variant Libraries

TCR Libraries NEW
Combinatorial Variant Library
Spread Out Low Diversity Libraries
Site Saturation Libraries
Enzyme Screening kits NEW

Antibodies

DISCOVERY
PRODUCTION AND CHARACTERIZATION

IVDR Solutions

Applications

AgBio

Support

Chinese
English
Japanese
Español

Support

Contact Us
FAQ
Match with an Expert

MY ORDERS

Open Orders
Drafts
Past Orders

NGS Workflow

NGS WORKFLOWS >

AI Drug Discovery

TOOLS FOR AI DRUG DISCOVERY

Cancer Research

Tools for Cancer Research and Clinical Oncology >
- Whole Exome Sequencing
- Whole Genome Sequencing
Liquid Biopsy >

AgBio

Drug Discovery

Tools for Drug Discovery
- VHH Antibody Discovery
- Bispecific antibodies
Antibody Services

Infectious Disease

Tools for Infectious Disease
SARS-CoV-2
- SARS-CoV-2 Research Panel
- SARS-CoV-2 RNA Controls
Catalog Antibodies
- Anti-ACE2
- Anti-SARS-CoV-2 S1
Synthetic Controls
- Monkeypox Virus Controls
- Respiratory Virus Controls
Fixed Panels
- Comprehensive Viral Research Panel
- Respiratory Virus Research Panel

resources

Technical Resources

Webinar

Case Studies

Publications

Podcasts

Videos

Blog

FAQ

Codon Optimization NEW

MasterMix Calculator

Twist Wallet

Support

Chinese
English
Japanese
Español

Support

Contact Us
FAQ
Match with an Expert

MY ORDERS

Open Orders
Drafts
Past Orders

Technical Resources

Webinar

Case Studies

Publications

Podcasts

Videos

Blog

FAQ

Codon Optimization NEW

MasterMix Calculator

Twist Wallet

COMPANY

Biopharma

Support

Chinese
English
Japanese
Español

Support

Contact Us
FAQ
Match with an Expert

MY ORDERS

Open Orders
Drafts
Past Orders

Company

About
Team
Careers
Investor Relations
Events
Contact
Blog
Corporate Responsibility

Technology

Overview
Ordering Platform
Twist API
Procurement Integrations

Investor Relations

Overview
STOCK
- Stock Quote Chart
- Analyst Coverage
News and Events
Financials
- Sec Filings
Governance
- Governance Hightlights
- Commitee Composition
Resources
- Email Alerts
- IR Contacts

Partnerships

NGS Prolab
Channel Partners and Distributors

Biopharma

Webinars

Validating a diagnostic cfDNA NGS assay

View the Webinar

Related Resources

All Resources View All

Pushing the Boundaries of High Sensitivity Epigenetic Applications

DNA methylation is an essential epigenetic modification that influences the control of gene expression. Anomalies in this process can lead to genomic instabilities or issues with transcriptional silencing, which can result in various diseases such as cancer, diabetes, cardiovascular disease, and various inflammatory conditions. Scientists must therefore access the right tools to effectively study a given sample’s DNA methylation status and better understand genome-wide methylation patterns.

Twist cfDNA Pan-Cancer Reference Standard Variant V2 List: in .vcf and Excel format | Twist Bioscience

Twist cfDNA Pan-Cancer Reference Standard Variant V2 List: in .vcf and Excel format | Twist Bioscience

All RESOURCES

INTEGRATIONS

TAPI
Procurement Integrations

Technology

Overview
Ordering Platform

Resources

Technical Resources
Webinars
Case Studies
Publications
Blog

Partnerships

NGS ProLab
Channel Partners & Distributors

Company

About
Team
Careers
Investor Relations
Events
Biopharma

Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080

Contact Us
FAQ

Terms & Conditions
Policy on Unsolicited Submissions of Information
Privacy Policy
Regulatory and Quality Information
Security
Payment Information
© 2025 Twist Bioscience. All rights reserved.