A key challenge exists whenever a new CRISPR application is developed: How do you predict which guide RNA sequence will maximize the chance of an edit? Prime editing is one of the newest CRISPR technologies. It is highly promising as it can install small and precise mutations with a unique reverse transcriptase dependent mechanism that eliminates the need for donor DNA and double-strand breaks. Due to its precise editing capabilities, it has potential applications in CRISPR therapeutics for treating genetic disease.  

The ability to generate 300mer oligos is a critical tool for researchers, enabling them to screen more than 70% of sequences identified in PFAM as human nuclear protein domains, which are potential effectors. Coupled with the exceptional precision, length, and uniformity of Twist Oligos, HT-recruit opens the door to protein domain characterization on a genome-wide scale.  

The use of CRISPR screening technology enables the interrogation of gene function at virtually any scale, especially in pooled screening applications. While genome-wide CRISPR screen libraries are easily accessible off-the-shelf, their lack of design flexibility means it is difficult to cost-effectively address specific research questions, thus limiting the applicability of these libraries. Twist Bioscience’s custom CRISPR guide libraries give you the flexibility to tailor your sgRNA library to your species, strain, and research question, all with the confidence that guides in your library will be precisely synthesized and equally represented.